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bj 5α  (ATCC)


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    ATCC bj 5α
    (A) Schematic of the single-vector V-SWITCH architecture. The CAM (EF1α promoter) encodes NLS–mNG3A(1–10)–PCS–ER anchor–T2A–blasticidin resistance. The NCM (CMV promoter) encodes NLS–mNG(11)–BFP–NLS. Both modules are delivered on a single lentiviral backbone. (B) Principle of single-vector reporter operation, as described for . In this design, the NCM is an exogenous NLS–mNG(11)–BFP fusion expressed from the CMV promoter. (C) Representative images of A549 DENV-SWITCH cells infected with DENV at increasing MOIs (uninfected, 0.3, 1, 5) and co-stained for NS3 (magenta). Reporter fluorescence (V-SWITCH) is shown in green. Scale bar, 20 µm. (D) Percentage of infected cells detected by anti-NS3 immunostaining (black bars) versus reporter fluorescence (white bars) across MOIs. Data are mean ± s.d. (n = 3 independent experiments). (E) Representative flow cytometry plots for DENV-SWITCH A549 cells infected at MOI 5. Left: reporter fluorescence (FITC-A); middle: NS3 immunostaining (PE-A); right: NS3 staining versus reporter fluorescence. BFP-positive cells were gated to define the reporter-expressing population. (F) Dose–response curve for MK0608 in DENV-SWITCH A549 cells. IC₅₀ is indicated. (G) Dose–response curve for niclosamide in DENV-SWITCH A549 cells. IC₅₀ is indicated. Data in (F–G) are mean ± s.d. (n = 3 independent experiments). (H) Effect of siRNA-mediated knockdown of EMC6 and RACK1 on DENV infection in A549 DENV-SWITCH cells at 24 hpi (MOI 5). DENV-infected cells are expressed as a percentage of siCTRL. ***P < 0.001, unpaired two-tailed t-test. Data are mean ± s.d. (n = 3 independent experiments). (I) Percentage of infected cells detected by anti-NS3 immunostaining (black bars) and reporter fluorescence (white bars) <t>in</t> <t>BJ-5α</t> fibroblasts infected with DENV at MOI 1, 5, and 10. Data are mean ± s.d. (J) Effect of HA15 (50 µM), a BiP/GRP78 ATPase domain inhibitor, on DENV infection in BJ-5α DENV-SWITCH cells at 24 hpi. DENV infection (black bars, % relative to DMSO control) and cell viability (white bars) are shown. ns, not significant; ***P < 0.001, unpaired two-tailed t-test. Data are mean ± s.d. (n = 3 independent experiments).
    Bj 5α, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bj 5α/product/ATCC
    Average 96 stars, based on 453 article reviews
    bj 5α - by Bioz Stars, 2026-06
    96/100 stars

    Images

    1) Product Images from "V-SWITCH: A single-vector OFF-to-ON fluorescent reporter of live RNA virus infections"

    Article Title: V-SWITCH: A single-vector OFF-to-ON fluorescent reporter of live RNA virus infections

    Journal: bioRxiv

    doi: 10.64898/2026.04.08.717260

    (A) Schematic of the single-vector V-SWITCH architecture. The CAM (EF1α promoter) encodes NLS–mNG3A(1–10)–PCS–ER anchor–T2A–blasticidin resistance. The NCM (CMV promoter) encodes NLS–mNG(11)–BFP–NLS. Both modules are delivered on a single lentiviral backbone. (B) Principle of single-vector reporter operation, as described for . In this design, the NCM is an exogenous NLS–mNG(11)–BFP fusion expressed from the CMV promoter. (C) Representative images of A549 DENV-SWITCH cells infected with DENV at increasing MOIs (uninfected, 0.3, 1, 5) and co-stained for NS3 (magenta). Reporter fluorescence (V-SWITCH) is shown in green. Scale bar, 20 µm. (D) Percentage of infected cells detected by anti-NS3 immunostaining (black bars) versus reporter fluorescence (white bars) across MOIs. Data are mean ± s.d. (n = 3 independent experiments). (E) Representative flow cytometry plots for DENV-SWITCH A549 cells infected at MOI 5. Left: reporter fluorescence (FITC-A); middle: NS3 immunostaining (PE-A); right: NS3 staining versus reporter fluorescence. BFP-positive cells were gated to define the reporter-expressing population. (F) Dose–response curve for MK0608 in DENV-SWITCH A549 cells. IC₅₀ is indicated. (G) Dose–response curve for niclosamide in DENV-SWITCH A549 cells. IC₅₀ is indicated. Data in (F–G) are mean ± s.d. (n = 3 independent experiments). (H) Effect of siRNA-mediated knockdown of EMC6 and RACK1 on DENV infection in A549 DENV-SWITCH cells at 24 hpi (MOI 5). DENV-infected cells are expressed as a percentage of siCTRL. ***P < 0.001, unpaired two-tailed t-test. Data are mean ± s.d. (n = 3 independent experiments). (I) Percentage of infected cells detected by anti-NS3 immunostaining (black bars) and reporter fluorescence (white bars) in BJ-5α fibroblasts infected with DENV at MOI 1, 5, and 10. Data are mean ± s.d. (J) Effect of HA15 (50 µM), a BiP/GRP78 ATPase domain inhibitor, on DENV infection in BJ-5α DENV-SWITCH cells at 24 hpi. DENV infection (black bars, % relative to DMSO control) and cell viability (white bars) are shown. ns, not significant; ***P < 0.001, unpaired two-tailed t-test. Data are mean ± s.d. (n = 3 independent experiments).
    Figure Legend Snippet: (A) Schematic of the single-vector V-SWITCH architecture. The CAM (EF1α promoter) encodes NLS–mNG3A(1–10)–PCS–ER anchor–T2A–blasticidin resistance. The NCM (CMV promoter) encodes NLS–mNG(11)–BFP–NLS. Both modules are delivered on a single lentiviral backbone. (B) Principle of single-vector reporter operation, as described for . In this design, the NCM is an exogenous NLS–mNG(11)–BFP fusion expressed from the CMV promoter. (C) Representative images of A549 DENV-SWITCH cells infected with DENV at increasing MOIs (uninfected, 0.3, 1, 5) and co-stained for NS3 (magenta). Reporter fluorescence (V-SWITCH) is shown in green. Scale bar, 20 µm. (D) Percentage of infected cells detected by anti-NS3 immunostaining (black bars) versus reporter fluorescence (white bars) across MOIs. Data are mean ± s.d. (n = 3 independent experiments). (E) Representative flow cytometry plots for DENV-SWITCH A549 cells infected at MOI 5. Left: reporter fluorescence (FITC-A); middle: NS3 immunostaining (PE-A); right: NS3 staining versus reporter fluorescence. BFP-positive cells were gated to define the reporter-expressing population. (F) Dose–response curve for MK0608 in DENV-SWITCH A549 cells. IC₅₀ is indicated. (G) Dose–response curve for niclosamide in DENV-SWITCH A549 cells. IC₅₀ is indicated. Data in (F–G) are mean ± s.d. (n = 3 independent experiments). (H) Effect of siRNA-mediated knockdown of EMC6 and RACK1 on DENV infection in A549 DENV-SWITCH cells at 24 hpi (MOI 5). DENV-infected cells are expressed as a percentage of siCTRL. ***P < 0.001, unpaired two-tailed t-test. Data are mean ± s.d. (n = 3 independent experiments). (I) Percentage of infected cells detected by anti-NS3 immunostaining (black bars) and reporter fluorescence (white bars) in BJ-5α fibroblasts infected with DENV at MOI 1, 5, and 10. Data are mean ± s.d. (J) Effect of HA15 (50 µM), a BiP/GRP78 ATPase domain inhibitor, on DENV infection in BJ-5α DENV-SWITCH cells at 24 hpi. DENV infection (black bars, % relative to DMSO control) and cell viability (white bars) are shown. ns, not significant; ***P < 0.001, unpaired two-tailed t-test. Data are mean ± s.d. (n = 3 independent experiments).

    Techniques Used: Plasmid Preparation, Infection, Staining, Fluorescence, Immunostaining, Flow Cytometry, Expressing, Knockdown, Two Tailed Test, Control



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    bj 5α  (ATCC)
    96
    ATCC bj 5α
    (A) Schematic of the single-vector V-SWITCH architecture. The CAM (EF1α promoter) encodes NLS–mNG3A(1–10)–PCS–ER anchor–T2A–blasticidin resistance. The NCM (CMV promoter) encodes NLS–mNG(11)–BFP–NLS. Both modules are delivered on a single lentiviral backbone. (B) Principle of single-vector reporter operation, as described for . In this design, the NCM is an exogenous NLS–mNG(11)–BFP fusion expressed from the CMV promoter. (C) Representative images of A549 DENV-SWITCH cells infected with DENV at increasing MOIs (uninfected, 0.3, 1, 5) and co-stained for NS3 (magenta). Reporter fluorescence (V-SWITCH) is shown in green. Scale bar, 20 µm. (D) Percentage of infected cells detected by anti-NS3 immunostaining (black bars) versus reporter fluorescence (white bars) across MOIs. Data are mean ± s.d. (n = 3 independent experiments). (E) Representative flow cytometry plots for DENV-SWITCH A549 cells infected at MOI 5. Left: reporter fluorescence (FITC-A); middle: NS3 immunostaining (PE-A); right: NS3 staining versus reporter fluorescence. BFP-positive cells were gated to define the reporter-expressing population. (F) Dose–response curve for MK0608 in DENV-SWITCH A549 cells. IC₅₀ is indicated. (G) Dose–response curve for niclosamide in DENV-SWITCH A549 cells. IC₅₀ is indicated. Data in (F–G) are mean ± s.d. (n = 3 independent experiments). (H) Effect of siRNA-mediated knockdown of EMC6 and RACK1 on DENV infection in A549 DENV-SWITCH cells at 24 hpi (MOI 5). DENV-infected cells are expressed as a percentage of siCTRL. ***P < 0.001, unpaired two-tailed t-test. Data are mean ± s.d. (n = 3 independent experiments). (I) Percentage of infected cells detected by anti-NS3 immunostaining (black bars) and reporter fluorescence (white bars) <t>in</t> <t>BJ-5α</t> fibroblasts infected with DENV at MOI 1, 5, and 10. Data are mean ± s.d. (J) Effect of HA15 (50 µM), a BiP/GRP78 ATPase domain inhibitor, on DENV infection in BJ-5α DENV-SWITCH cells at 24 hpi. DENV infection (black bars, % relative to DMSO control) and cell viability (white bars) are shown. ns, not significant; ***P < 0.001, unpaired two-tailed t-test. Data are mean ± s.d. (n = 3 independent experiments).
    Bj 5α, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bj 5α/product/ATCC
    Average 96 stars, based on 1 article reviews
    bj 5α - by Bioz Stars, 2026-06
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic of the single-vector V-SWITCH architecture. The CAM (EF1α promoter) encodes NLS–mNG3A(1–10)–PCS–ER anchor–T2A–blasticidin resistance. The NCM (CMV promoter) encodes NLS–mNG(11)–BFP–NLS. Both modules are delivered on a single lentiviral backbone. (B) Principle of single-vector reporter operation, as described for . In this design, the NCM is an exogenous NLS–mNG(11)–BFP fusion expressed from the CMV promoter. (C) Representative images of A549 DENV-SWITCH cells infected with DENV at increasing MOIs (uninfected, 0.3, 1, 5) and co-stained for NS3 (magenta). Reporter fluorescence (V-SWITCH) is shown in green. Scale bar, 20 µm. (D) Percentage of infected cells detected by anti-NS3 immunostaining (black bars) versus reporter fluorescence (white bars) across MOIs. Data are mean ± s.d. (n = 3 independent experiments). (E) Representative flow cytometry plots for DENV-SWITCH A549 cells infected at MOI 5. Left: reporter fluorescence (FITC-A); middle: NS3 immunostaining (PE-A); right: NS3 staining versus reporter fluorescence. BFP-positive cells were gated to define the reporter-expressing population. (F) Dose–response curve for MK0608 in DENV-SWITCH A549 cells. IC₅₀ is indicated. (G) Dose–response curve for niclosamide in DENV-SWITCH A549 cells. IC₅₀ is indicated. Data in (F–G) are mean ± s.d. (n = 3 independent experiments). (H) Effect of siRNA-mediated knockdown of EMC6 and RACK1 on DENV infection in A549 DENV-SWITCH cells at 24 hpi (MOI 5). DENV-infected cells are expressed as a percentage of siCTRL. ***P < 0.001, unpaired two-tailed t-test. Data are mean ± s.d. (n = 3 independent experiments). (I) Percentage of infected cells detected by anti-NS3 immunostaining (black bars) and reporter fluorescence (white bars) in BJ-5α fibroblasts infected with DENV at MOI 1, 5, and 10. Data are mean ± s.d. (J) Effect of HA15 (50 µM), a BiP/GRP78 ATPase domain inhibitor, on DENV infection in BJ-5α DENV-SWITCH cells at 24 hpi. DENV infection (black bars, % relative to DMSO control) and cell viability (white bars) are shown. ns, not significant; ***P < 0.001, unpaired two-tailed t-test. Data are mean ± s.d. (n = 3 independent experiments).

    Journal: bioRxiv

    Article Title: V-SWITCH: A single-vector OFF-to-ON fluorescent reporter of live RNA virus infections

    doi: 10.64898/2026.04.08.717260

    Figure Lengend Snippet: (A) Schematic of the single-vector V-SWITCH architecture. The CAM (EF1α promoter) encodes NLS–mNG3A(1–10)–PCS–ER anchor–T2A–blasticidin resistance. The NCM (CMV promoter) encodes NLS–mNG(11)–BFP–NLS. Both modules are delivered on a single lentiviral backbone. (B) Principle of single-vector reporter operation, as described for . In this design, the NCM is an exogenous NLS–mNG(11)–BFP fusion expressed from the CMV promoter. (C) Representative images of A549 DENV-SWITCH cells infected with DENV at increasing MOIs (uninfected, 0.3, 1, 5) and co-stained for NS3 (magenta). Reporter fluorescence (V-SWITCH) is shown in green. Scale bar, 20 µm. (D) Percentage of infected cells detected by anti-NS3 immunostaining (black bars) versus reporter fluorescence (white bars) across MOIs. Data are mean ± s.d. (n = 3 independent experiments). (E) Representative flow cytometry plots for DENV-SWITCH A549 cells infected at MOI 5. Left: reporter fluorescence (FITC-A); middle: NS3 immunostaining (PE-A); right: NS3 staining versus reporter fluorescence. BFP-positive cells were gated to define the reporter-expressing population. (F) Dose–response curve for MK0608 in DENV-SWITCH A549 cells. IC₅₀ is indicated. (G) Dose–response curve for niclosamide in DENV-SWITCH A549 cells. IC₅₀ is indicated. Data in (F–G) are mean ± s.d. (n = 3 independent experiments). (H) Effect of siRNA-mediated knockdown of EMC6 and RACK1 on DENV infection in A549 DENV-SWITCH cells at 24 hpi (MOI 5). DENV-infected cells are expressed as a percentage of siCTRL. ***P < 0.001, unpaired two-tailed t-test. Data are mean ± s.d. (n = 3 independent experiments). (I) Percentage of infected cells detected by anti-NS3 immunostaining (black bars) and reporter fluorescence (white bars) in BJ-5α fibroblasts infected with DENV at MOI 1, 5, and 10. Data are mean ± s.d. (J) Effect of HA15 (50 µM), a BiP/GRP78 ATPase domain inhibitor, on DENV infection in BJ-5α DENV-SWITCH cells at 24 hpi. DENV infection (black bars, % relative to DMSO control) and cell viability (white bars) are shown. ns, not significant; ***P < 0.001, unpaired two-tailed t-test. Data are mean ± s.d. (n = 3 independent experiments).

    Article Snippet: A549 (ATCC, CCL-185), HEK 293T (ATCC, CRL-3216), HEK 293FT (Invitrogen, R70007), and BJ-5α (ATCC, CRL-4001) cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with 10% foetal bovine serum (FBS; Gibco) at 37°C in a humidified atmosphere containing 5% CO2.

    Techniques: Plasmid Preparation, Infection, Staining, Fluorescence, Immunostaining, Flow Cytometry, Expressing, Knockdown, Two Tailed Test, Control